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. 2019 Jul 31;4(4):e00276-19. doi: 10.1128/mSphere.00276-19

FIG 3.

FIG 3

A wild-type copy of ROP17 rescues the Myr− phenotype of the Δrop17 mutant. (A) Strategy for complementing the Δrop17 mutants with a 3xHA-tagged wild-type copy of ROP17. (B) IFA showing successful complementation of the Δrop17 mutant with a HA-tagged wild-type copy of the gene. Green shows staining with anti-ROP2/3/4 as a marker for rhoptries; red shows staining for the complementing ROP17-HA. (C) IFA of HFFs infected with RH-WT, RHΔrop17, or RHΔrop17::ROP17-3xHA. Anti-HA antibody was used to detect the complementing ROP17 (green), while red was used for staining of host c-Myc as an indicator of successful effector translocation, and blue shows DAPI staining of the host nuclei. Hollow white arrows indicate parasitophorous vacuoles; solid white arrows indicate the host nuclei in infected cells (quantitation of similar experiments is shown in Fig. 5).