FIG 6.

ROP17’s role in effector translocation occurs at the host cytosolic side of the PVM. (A) Conditions used for coinfection of host cells with RHΔrop17::GRA24MYC and RHΔmyr1 expressing mCherry. Infections were initiated with the indicated strain, followed by addition of the second strain 1 h later, followed by IFA after a further 17 h. (B) Quantitation of the percentage of host nuclei staining positive for GRA24MYC in the cells infected with the indicated strains. Cells infected with one or other of the two mutants showed no GRA24MYC, whereas those that were coinfected with both mutants showed substantial rescue in “trans.” Data shown are averages of technical replicates and are representative of two independent biological replicates. Differences were assessed by ANOVA with a post hoc Tukey’s test. **, P < 0.001.