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. 2019 Jul 30;12(10):1375–1385. doi: 10.1016/j.tranon.2019.07.007

Figure 3.

Figure 3

JAG agents mediate a potent anti-pHGG response with limited toxicity to non-neoplastic astrocytes. (A) KNS42 and (B) SF188 pHGG cells treated with each JAG agent. After 96 hours, loss of cell viability was determined by CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). Representative microscopy (×10) images of (C) KNS42 and SF188 cells. Cells were treated with indicated agents (30 μM per JAG agent) and imaged (up to 24 hours) at 37°C under normoxic conditions. Images and films were recorded using EVOS FL Auto (Life Technologies). (D) Apoptosis assays were conducted for each indicated cell line 24 hours post 30 μM treatment with each JAG agent. Annexin V/PI staining was conducted, and apoptosis status was determined using an NC-3000 counter; n = 3 ± SD. (E and F) Dose-response curves for non-neoplastic CC2565 cells following exposure to TMZ (≤128 mM), vincristine (≤1 mM), or individual JAG agents ≤1000 μM in complete astrocyte growth medium. Data presented as average of n = 3. Error bars indicate ±SD.