Table 1. The biogenetic characteristics of IL-6, IL-18 and GAPDH transcripts .
| Gene | Primer sequences (5'→3') | PCR product length | Thermocycling condition |
|
IL-6:F IL-6:R |
ACCCCCAATAAATATAGGACTGGA GCTTCTCTTTCGTTCCCGGT |
101 bp | 95°C/2 min, 40 cycles of 95°C/30 s, 60°C/20 s and 70°C/30 s |
|
IL-18:F IL-18:R |
AGCTTGTGAAAAAGAGAGAGACCT GCTAGTCTTCGTTTTGAACAGTGA |
75 bp | 95°C/2 min, 40 cycles of 95°C/30 s, 60°C/20 s and 70°C/30 s |
|
GAPDH:F GAPDH:R |
GGACTCATGACCACAGTCCA CCAGTAGAGGCAGGGATGAT |
119 bp | 95°C/2 min, 40 cycles of 95°C/30 s, 57.5°C/20 s and 70°C/30 s |
PCR: Polymerase Chain Reaction, F: Forward primer, R: Reverse primer, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase, bp: base pairs.