Table 1. Primer sequences and their product size .
SNP | Primer name | Primer sequence (5'-3') | Ta (°C) | Amplicon size (bp) |
rs2373929 | FO | CCCGCTGTTTCTGCTTTTC | 60 |
FO-RO: 426 FO-RI [allele T]: 307 RO-FI [allele C]: 157 |
RO | TCTTTGCTCCCATTTACTGAC | |||
FI | TTCCTGTGCTCTCGTGACC | |||
RI | GTTGCCATCTGACAGACTAA | |||
rs7830 | FO | GACATCGCAGTCCCCTTCC | 59 |
FO-RO: 833 Allele specific: 390 |
RO | TGAGTCATCTAAGTATTCTTCAATCCAA | |||
FI C | CCTTCAGGCAGTCCTTTAATCC | |||
FI A | CTTCAGGCAGTCCTTTGGTCA |
SNP alleles show in bold and underline.