Figure 2.

Assay specificity and cross-reactivity. SEA induced a specific reduction in TCR Vβ9 protein. CCRF-CEM T-cells in combination with Raji B cells were incubated for 2 hours with 1 µg/mL of SEA, SEB, SED or SEE. Cells were stained with PE-conjugated anti-Vβ9 monoclonal antibody (mAb) after stimulation. Fluorescence intensity was measured by flow cytometry. Data are representative of three independent experiments showing the mean florescence for the live T-cell population (about 8000 cells).