Figure 3.

SEA induces reduction in TCR Vβ9 protein in a dose-dependent manner and can discriminate between biologically-active and inactive SEA. The mixed CCRF-CEM T-cells and Raji B cells were co-incubated with increasing concentrations of SEA for 2 h. After stimulation, the cells were stained with PE-conjugated anti-Vβ9 mAb. Mean fluorescence intensity for Vβ9 in live T-cell population was measured by flow cytometry. Data are representative of three independent experiments.