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. 2019 Jul 17;11(7):651. doi: 10.3390/v11070651

Figure 8.

Figure 8

MOV10 over-expression does not induce the degradation of HBV pgRNA. 293T cells were transfected with HBV core-deficient plasmid (Cp-deficient pHBV) and peYFP-MOV10 or peYFP-empty (eYFP) plasmids. After 48 h, the cells were treated with 5 µg/mL Actinomycin D. The cells were harvested at 0, 2, 4, 6 h after Actinomycin D treatment. Total RNA was extracted and HBV pgRNA levels were determined relative to the 0 h time point. RPL18A mRNA was used as a normalization control. Single phase decay curves are shown. The dotted line represents cells transfected with Cp-deficient pHBV and peYFP in the absence of Actinomycin D. The solid black line represents cells transfected with Cp-deficient pHBV and peYFP in the presence of Actinomycin D. The grey line represents cells transfected with Cp-deficient pHBV and peYFP-MOV10 in the presence of Actinomycin D. Data represent mean ± standard deviation from two independent experiments.