Table 1.
Name of the Primer | Nucleotide Sequence | PCR Product Size |
---|---|---|
RT-PCR | ||
PuuV-For 1st round | 5′-CTGCAAGCCAGGCAACAAACAGTGTCAGCA-3′ | 723 bp |
PuuV-Rev 1st round | 5′-GTCTGCCACATGATTTTTGTCAAGCACATC-3′ | |
PuuV-For 2st round | 5’-AAGTGGCCAGACAGCAGATT-3’ | 217 bp |
PuuV-Rev 2st round | 5’-GGCAGTAGGCATGGAAACAT-3’ | |
qPCR | ||
PuuVN-F | 5′-AGGCAACAAACAGTGTCAGC-3′ | 186 bp |
PuuVN-R | 5′-AATCCCAGTCGGGTCAGTAG-3′ | |
GAPDH-F | 5′-TTTGGCTACAGCAACAGG-3′ | 107 bp |
GAPDH-R | 5′-GGTCTCTCTCTTCCTCTTG-3′ |