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. 2019 Jul 2;11(7):601. doi: 10.3390/v11070601

Table 1.

Primers used in the study.

Name of the Primer Nucleotide Sequence PCR Product Size
RT-PCR
PuuV-For 1st round 5′-CTGCAAGCCAGGCAACAAACAGTGTCAGCA-3′ 723 bp
PuuV-Rev 1st round 5′-GTCTGCCACATGATTTTTGTCAAGCACATC-3′
PuuV-For 2st round 5’-AAGTGGCCAGACAGCAGATT-3’ 217 bp
PuuV-Rev 2st round 5’-GGCAGTAGGCATGGAAACAT-3’
qPCR
PuuVN-F 5′-AGGCAACAAACAGTGTCAGC-3′ 186 bp
PuuVN-R 5′-AATCCCAGTCGGGTCAGTAG-3′
GAPDH-F 5′-TTTGGCTACAGCAACAGG-3′ 107 bp
GAPDH-R 5′-GGTCTCTCTCTTCCTCTTG-3′