Host binding assay of GFP-labelled TpeX of the MTP of Phi2R06A against eight strains: four hosts which the mature phage is capable of infecting (A–D), and four non-host strains (E–H). Binding to host strain L. lactis 2 (CWPS Type B; panel A), host strain L. lactis 10 (CWPS Type B; panel B), host strain L. lactis 17 (CWPS Type B; panel C), host strain L. lactis 3 (CWPS Type Unknown; panel D), non-host strain L. lactis C (CWPS Type A; panel E), non-host strain L. lactis 4 (CWPS Type C; panel F), non-host strain L. lactis 13 (CWPS Type C; panel G), non-host strain L. lactis A (CWPS Type Unknown, panel H). MTP was added at a quantity of 25 μg. Cells were visualised using differential interference contrast (DIC) microscopy (panels on the left), and fluorescent confocal microscopy (panels on the right) at wavelength of 488 nm. Scale bars correspond to 10 μm.