Table 3.
Validation parameters of the established methods.
| Mouse Recovery (% of Expected Result) | LOD (ng/mL) | LOQ (ng/mL) | Linear Range (ng/mL) | ||
|---|---|---|---|---|---|
| Congener | Mouse Serum | Mouse Liver | Mouse Serum | Mouse Serum | Mouse Serum |
| MC-RR | 74.2% | 78.7% | 0.5 | 2 | 2 - 500 |
| MC-YR | 88.5% | 82.9% | 0.5 | 2 | 2 - 500 |
| MC-LR | 113.1% | 81.2% | 0.1 | 2 | 2 - 500 |
| MC-FR | 124.3% | 86.3% | - | - | - |
| MC-WR | 108.6% | 94.5% | - | - | - |
| MC-RA | 111.2% | 120.9% | - | - | - |
| MC-RAba | 106.4% | 109.6% | - | - | - |
| MC-LA | 86.1% | 64.5% | - | - | - |
| MC-FA | 100.0% | 43.4% | - | - | - |
| MC-WA | 96.8% | 34.1% | - | - | - |
| MC-LAba | 94.3% | 42.2% | - | - | - |
| MC-FAba | 97.4% | 54.4% | - | - | - |
| MC-WAba | 89.8% | <LOQ | - | - | - |
| MC-LF | 83.5% | 90.6% | (0.5) | (2) | (2 - 500) |
| D7-MC-LR | 100.0% | 100.0% | - | - | - |
| D5-MC-LF | 100.0% | 100.0% | - | - | |
MC-RR, MC-YR and MC-LR were used as external standards. MC-LF was also part of the external standard mix, but was not used to quantify any of the other MC congeners as it was no certified reference material but served as reference for more hydrophobic congeners. D7-MC-LR and D7-MC-LR were used as internal standards in every sample quantified. Recovery was calculated using the external standards in MeOH. Therefore, the recovery as calculated here shows the influence of the matrix. MC congener mix spiked mouse plasma and liver homogenates from untreated male animals were used for this experiment. Linear regressions for the linearity determination all showed R2 > 0.99.