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. 2019 Jul 8;9(7):986. doi: 10.3390/nano9070986

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Enhanced green fluorescent protein (eGFP) knockdown of NM_0.2/CP_0.8 polyplexes in human non-small cell lung carcinoma cells expressing eGFP (H1299/eGFP) quantified by flow cytometry as median fluorescence intensity (MFI) of eGFP after transfection with polyplexes at N/P 4 with eGFP siRNA or scrambled control siRNA (siNC) for 48 h with and without chloroquine treatment. Blank samples consisted of H1299/eGFP untreated cells. The positive control consisted of Lipofectamin (LF) 2000 lipoplexes formulated with eGFP siRNA or scrambled control siRNA with and without chloroquine treatment (data points indicate mean ± SD, n = 3, two-way ANOVA with Bonferroni post-hoc test, ** p < 0.01, *** p < 0.005).