Table 1.

Particle diameters of NM_0.2/CP_0.8 polyplexes at N/P ratios of 4, 5.5, 7.5, and 11.5 diluted 1X with electrolyte solution (30 mM of (4-(2-hydroxyethyl)–1-piperazineethanesulfonic acid) (HEPES), 100 mM of potassium chloride, 2 mM of EDTA, and 0.03% Tween^®20) obtained by dynamic light scattering (DLS) and tunable resistive pulse sensing (TRPS) measurements (data shown as mean ± standard deviation, n = 3).

N/P Ratio	DLS (Mean Size ± SD) [nm]	TRPS (Mean Size ± SD) [nm]
4	158.00 ± 5.18	177.00 ± 15.56
5.5	133.37 ± 1.79	100.50 ± 0.71
7.5	144.30 ± 1.76	164.00 ± 22.63
11.5	164.00 ± 3.8	193.00 ± 5.66