Table 2.
Purification of antibacterial peptides from hydrolysate of whole mackerel.
| Purification Step | Volume (mL) | Total Protein (mg) | Total Activity (AU) | Specific Activity (AU/mg) | Increase in Specific Activity (fold) | Activity Recovery (%) |
|---|---|---|---|---|---|---|
| Solubilized hydrolysate | 50 | 1.25 × 103 | 1.00 × 106 | 8.00 × 101 | / | 100 |
| SPE-C18 eluate | 1.0 | 1.48 × 100 | 6.40 × 105 | 4.32 × 105 | 5405 | 64 |
| First RP-HPLC eluate | 0.5 | 1.70 × 10−2 | 2.00 × 104 | 1.18 × 106 | 14,706 | 2 |
| Second RP-HPLC eluate | 0.3 | 4.00 × 10−3 | 6.00 × 103 | 1.67 × 106 | 20,833 | 1 |