Table 2.

Summary of findings for diagnostic assays used in the experimental inoculation experiments. Bold font indicates that at least one cat was positive for the measured value, or differences in values between naïve and FFV-infected animals were significant. Cat FFV5 was on a different inoculation and sample collection schedule following re-inoculation on day 53 pi (Figure 1).

Assay	Days Tested	Summary of Findings
Saliva qPCR (RNA)	36, 42, 49, 56, 63, 86, 112, 119, 126, 133, 142, 147, 154, 161, 168, 176	D133 saliva sample was FFV DNA-negative.
Plasma qPCR (DNA)	42, 86, 142, 176	FFV not detected.
Plasma qPCR (RNA)	15, 56, 112	FFV not detected.
Tissue qPCR (DNA)	176	Virus shows primarily lymphoid tropism. Cat FFV3 had expanded tropism to other lymphoid and non-lymphoid tissues (Table 3). FFV not detected in cat FFV4’s tissues.
CBC, Chemistry	−21, 0, 3, 7, 10, 15, 21, 28, 42, 56, 63, 70, 77, 86, 98, 112, 126, 142, 154, 168, 176	Not indicative of disease.
PBMC Phenotype	−21, 0, 3, 7, 10, 15, 21, 28, 42, 56, 70, 86, 112, 142, 168	Significantly increased populations in FFV cats included monocytes and CD21+MHCII+, while CD8+CD25+, CD8+CD134+, CD8+FAS+, CD56+, and CD56+MHCII+ cells were decreased.
BUN, Creatinine	−21, 0, 7, 15, 21, 28, 42, 56, 63, 70, 77, 86, 98, 112, 126, 142, 154, 168, 176	While BUN remained within normal limits for all cats, values were significantly increased in FFV group cats compared to naïve on bolded days. Creatinine values were within normal ranges and did not rise above 1.8 mg/dl.
Urinalysis	−21, 3, 7, 10, 42, 56, 63, 70, 86, 91, 98, 112, 122, 142, 156, 171, 176	USG > 1.035 for all cats. Urinalysis and urine sediment were unremarkable.
UPC Ratio	36, 70, 86, 91, 98, 122, 142, 156, 171, 176	UPC ratio was 0.1 (normal) for all cats, except cat FFV3 where it increased to 0.2 (borderline proteinuric) on 122 and 142 days pi, during highest PBMC proviral load for this cat [10].