Figure 7.

An overview of the endopeptidase mass spectrometry assay, serotype specific antibodies are conjugated to magnetic beads then added to a sample (A); the beads are removed and thoroughly washed before a substrate that mimics the toxins natural target is added (B). The solution is then incubated, and the resulting mixture is analysed by mass spectrophotometry. Both whole substrate and cleaved fragments that result from incubation with BoNT can be detected (C).