Table 1.

Maximum value of polarization shift (∆P_max) obtained at the optimized antibody concentrations for each antibody/tracer combination.

MAb	Clone	[MAb] (µg/mL)	∆Pmax (mP) 1
			T2-FL (Dilution 1:3600) 2	HT2-FL1a (Dilution 1:3000) 2	HT2-FL1b (Dilution 1:400) 2	HT2-FL2 (Dilution 1:3600) 2
Anti-T2G	1–2	190	138 3	-	59	57
	1–3	40	280 3	39	262 3	159 3
	1–4	104	225 3	28	253 3	176 3
	2–5	89	116	53	246 2	27
	2–11	118	144 3	18	50	18
	2–13	134	191 3	23	28	19
	2–16	90	200 3	37	82	21
	2–17	120	129	27	156 3	18
	2–21	132	159 3	22	14	16
	2–44	155	176 3	133 3	246 3	49
Anti-T2	1	6	282 3	217 3	93	111
Anti-HT2	H10-A10	8	205 3	230 3	122	20

¹ ∆P_max = mP_MAb − mP_tracer; −∆P_max < 10mP; ² Optimised dilution (v/v) of the stock solutions providing a total fluorescence intensity equal to 3-fold the blank signal measured for PBS-A; ³ selected antibody/tracer combinations.