Table 3.
Statistical performances of the single-laboratory validation over 5 days of the FPIA for the determination of T-2, HT-2, T-2G and HT-2G (expressed as sum) with blank and artificially contaminated (at the screening target concentration of 100 µg/kg) wheat samples. Cut-off levels and rate of false suspect results were calculated according to the Regulation (EU) No. 519/2014.
| Performances | Protocol A | Protocol B | ||
|---|---|---|---|---|
| Blank | STC 1 (100 µg/kg) |
Blank | STC 1 (100 µg/kg) |
|
| Mean value 2 (µg/kg) | 12 | 115 | 21 | 104 |
| RSDr 3 (%) | 16 | 5 | 14 | 9 |
| RSDRI 4 (%) | 25 | 10 | 16 | 13 |
| Cut-off level | 96 | 80 | ||
| Rate of false suspect results (%) | <0.1 | <0.1 | ||
1 STC, screening target concentration; 2 The mean value of the total content of T-2, HT-2, T-2G and HT-2G (µg/kg, expressed as sum) (n = 20 replicates); 3 RSDr, relative standard deviation of the repeatability; 4 RSDRI, relative standard deviation (intermediate precision).