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. 2019 Jul 25;10:1738. doi: 10.3389/fimmu.2019.01738

Figure 1.

Figure 1

Akt3Nmf350 mice have a lag in disease onset and a less severe EAE course. (A) EAE disease course of male and female Akt3Nmf350 (n = 24) vs. WT (n = 24) mice. (#) Represents day of peak disease severity WT: day 14 (CI = 2.1) vs. Akt3Nmf350: day 20 (CI = 1.6) post-MOG immunization. (B) Day of onset of EAE symptoms (CI ≥ 1) in Akt3Nmf350 (n = 56) and WT (n = 52). (C) Histological analysis of representative sections of lumbar spinal cords from WT (n = 4) and Akt3Nmf350 (n = 6) mice after 10 days with consecutive clinical scores (chronic EAE) incubated with MBP (top row) and SMI32 (bottom row) are depicted. (a,b) MBP immunostaining quantified in (D) and (c,d) SMI32 immunostaining quantified in (E). Quantification of the SMI32+ axonal swellings (>3 μm) was conducted on multiple 20× fields of the left and the right ventral region of the lumbar spinal cord (*p = 0.05, ***p = 0.001, Mann-Whitney U-test). All scale bars are 500 μm.