Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 25;10:1738. doi: 10.3389/fimmu.2019.01738

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 DuBois, Ray, Gruber, Zhang, Aflakpui, Macian-Juan and Shafit-Zagardo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CD4-CKO mice have increased pro-inflammatory cytokine expression and decreased FOXP3 expression during chronic EAE. Protein was extracted from a 2 mm section of the corpora callosa of chronically ill mice. (A) Protein homogenates were prepared from CD4-CKO and WT (CD4Cre) mice, and protein levels of inflammatory cytokines measured by ELISA (pg/mg total protein) (n = 8–11). (B) Protein levels of inflammatory cytokines in the spinal cord measured by ELISA (pg/mg total protein) (n = 6). (C) Spinal cord mRNA expression of FOXP3 measured by qRT-PCR (n = 6–10). All genes were normalized to HPRT expression. Differences in expression are shown as 2^−ΔΔCt. Data were analyzed using 2-way ANOVA, *p < 0.05 is considered statistically significant.