Table 2.

Enzymes of the α-amidation pathway detected in scorpions.

Family	Species	PAM	PHM	PAL	PC1	PC2	CPE
Buthidae	Centruroides sculpturatus	◼-◼	◼	◼	⬤	⬤	⬤
	Centruroides hentzi	◼-◼	◼	◼	⬤		⬤
	Centruroides noxius a	◼-◼	◼	◼	⬤		⬤
	Centruroides limpidus b	◼-◼	◼	◼	⬤	⬤	⬤
	Centruroides orizaba	◼-◼	◼	◼	⬤	⬤	⬤
	Centruroides ochraceus	◼-◼	◼	◼	⬤	⬤	⬤
	Centruroides hirsutipalpus	✓	◼	◼	⬤		⬤
	Tityus trivittatus	◼-◼	◼	◼	⬤	⬤	⬤
	Leiurus abdullahbayrami *	◼-◼	◼	◼	⬤	⬤	⬤
	Mesobuthus martensii *	◼-◼	◼	◼
Vaejovidae	Thorellius cristimanus	◼-◼	◼	◼	⬤	⬤	⬤
	Paravaejovis schwenkmeyeri	✓	◼	◼		⬤	⬤
	Chihuahuanus coahuilae	◼-◼	◼	◼	⬤	⬤	⬤
	Serradigitus gertschi	✓	◼	◼
Caraboctonidae	Hoffmannihadrurus aztecus	◼-◼	◼	◼
	Hadrurus concolorus	◼-◼	◼	◼		✓	⬤
Euscorpiidae	Megacormus gertschi	◼-◼	◼	◼
Chactidae	Anuroctonus pococki bajae	✓	◼	◼
Superstitionidae	Superstitionia donensis	✓	◼	✓		✓
Diplocentridae	Diplocentrus melici	◼-◼	◼	◼		⬤
Urodacidae	Urodacus yaschenkoi *	◼-◼	◼	◼	⬤	✓	⬤
Scorpionidae	Pandinus imperator *	◼-◼	◼			⬤

(◼-◼, ◼ and ◼): Complete PAM, PHMm and PALm sequences; (◼-◼): Partial PAM sequences with 93% or more of the sequence determined; (⬤, ⬤, ⬤): Complete PC1, PC2 and CPE sequences; (⬤, ⬤, ⬤): PC1, PC2 and CPE sequences with more than 50% of the sequence determined; (✓): Partial sequences with less than 50% of the estimated total sequence determined; ^a PAM sequence amplified by PCR; ^b PAM sequence verified by DNA sequencing; * Old World scorpion. The tblast and blastn algorithms were used to identify sequences in the local scorpion transcriptomic databases, with an e-value of 1 × 10⁻⁶. Empty spaces indicate that no sequences were identified in those transcriptomes.