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Membrane incorporation assay. Test for proper insertion and orientation of viral chimera based on the malE- complementation assay [62]. Bacteria lacking any maltose binding protein (MBP) were transformed with various viroporins which were fused to MBP and cultivated on M9 agar containing 0.4% maltose and 10 μM isopropyl β-d-1-thiogalactopyranoside (IPTG).
