Table 2.

Transmembrane segment prediction of the candidate viroporins analyzed in the current study. See Table 1 for complete names of proteins and accession numbers. All programs were run with default values.

Proteins	Phobious [39,40]	TMpred [41]	MEMSAT [42]
EEEV 6k protein	17–33; 38–56	16–37; 38–57	No TM detected
WNV MgM	42–70	42–75	41–58
DV Mg	44–70	41–75	44–68
DV 2k	6–23	8–24	Too short for prediction
VV gp151	12–32	12–32	12–32
VV gp170	30–57	39–57	39–57