Figure 1.

Schematic representation of the West Nile Virus (WNV) N-terminal polyprotein with numbered amino acids (one-letter codes; beginning from the N-terminal (H₂N) methionine (M)) and predicted transmembrane domains (bold numbers 1–7). The gE sequence is presented in blue with its neutralization domain III in yellow. NS1 is depicted in grey. External parts of the sequence (labeled extra) are depicted above the symbolic membrane and cytoplasmic parts are shown below (labeled intra). For producing immunizing antigens, the external domains of gE and NS1, respectively, were bracketed each with a synthetic N-terminal signal sequence and two C-terminal tag epitopes (V5 and 6× his). For the ELISA antigens, the DIII domain of gE was supplemented with the synthetic N-terminal signal sequence and with a C-terminal c-myc-GST-tail. Similarly, the NS1 fragment from aa 792 to 1050 was bracketed with the same features. (Picture exported from PROTTER, Protter: interactive protein feature visualization and integration with experimental proteomic data) [49].