Figure 2.

Cultures of human primary pancreatic ductal cells inoculated with CV-B4 E2. Ductal cell cultures were mock-infected or infected with CV-B4 E2. (A) The cell layer was observed under an inverted microscope 72 h post-inoculation: (a) mock-infected (b) infected with CV-B4 E2 (magnification: 40×). (B) The viral titers in culture supernatants harvested 3 h and 72 h post-inoculation were determined by plaque infectivity assay using HEp-2 cells. Results are mean + SD of five independent experiments. The limit of detection of the assay is represented by a dashed line.