Table 1.
Temperature dependence of the hydrodynamic diameter, polydispersity index (PDI), and zeta potential of fusogenic (DOPE/DOTAP/TFPE-head 1/1/0.1 mol/mol) (FLs) and endocytic (DOPC/DOTAP/TFPE-head 1/1/0.1 mol/mol) (ELs) liposomes. Experiments are performed in a PBS buffer at physiological pH 7.4. Given are averages over three independent measurements and, in parentheses, their standard deviations.
| Liposomal Type | Hydrodynamic Diameter (nm) (s.d.) | |||
|---|---|---|---|---|
| 4 °C | 20 °C | 30 °C | 37 °C | |
| (FLs) | 110 (13) | 116 (13) | 118 (16) | 117 (13) |
| (ELs) | 142 (42) | 154 (68) | 158 (71) | 155 (72) |
| PDI (s.d.) | ||||
| (FLs) | 0.26 (0.06) | 0.23 (0.02) | 0.23 (0.02) | 0.23 (0.02) |
| (ELs) | 0.34 (0.12) | 0.32 (0.11) | 0.35 (0.11) | 0.33 (0.11) |
| Zeta Potential (mV) (s.d.) | ||||
| (FLs) | 59 (4) | 48 (8) | 51 (3) | 43 (13) |
| (ELs) | 68 (13) | 64 (10) | 56 (8) | 58 (8) |