Table 2.
Temperature dependence of fusion efficiencies of liposomes containing the cationic lipid DOTAP, different neutral lipids, and TFPE-head as an aromatic molecule (1/1/0.1 mol/mol). Experiments are performed in a PBS buffer at physiological pH 7.4 and osmolality 280 mOsm/kg. Given are averages over at least three independent measurements and, in parentheses, their standard deviations.
| Liposomal Composition | Fusion Efficiency % (s.d.) | |||
|---|---|---|---|---|
| 4 °C | 20 °C | 30 °C | 37 °C | |
| C16(0)PE/DOTAP/TFPE-head | 97 (1) | 98 (2) | 98 (1) | 99 (1) |
| C16(0)PC/DOTAP/TFPE-head | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| C16(1)PE/DOTAP/TFPE-head | 88 (11) | 93 (6) | 96 (3) | 98 (2) |
| C16(1)PC/DOTAP/TFPE-head | 13 (3) | 16 (7) | 14 (3) | 17 (1) |
| C18(0)PE/DOTAP/TFPE-head | 92 (6) | 96 (1) | 89 (9) | 95 (5) |
| C18(0)PC/DOTAP/TFPE-head | 37 (8) | 38 (11) | 47 (6) | 62 (5) |
| C18(1)PE/DOTAP/TFPE-head | 83 (9) | 90 (3) | 87 (4) | 92 (6) |
| C18(1)PC/DOTAP/TFPE-head | 3 (2) | 5 (4) | 2 (1) | 4 (2) |