Figure 1.

Illustration of starvation conditions. (a) Short-term starvation: Cells were seeded in standard medium. On day 1, medium was changed to starvation medium (i.e., starvation medium with variations in contents of glucose and fetal calf serum (FCS)). On day 2, infection with MeV was performed in infection medium (Opti-MEM^®). At 3 h post-infection (hpi), infection medium was replaced with standard medium. At 96 hpi, the remaining tumor cell masses were determined by the sulforhodamine B (SRB) assay. (b) Long-term starvation: Cells were seeded in standard medium on day 0. Medium was changed to starvation medium on day 1. The 3 hpi, infection medium was replaced with the respective starvation medium. At 96 hpi, the remaining tumor cell masses were determined by SRB or lactate dehydrogenase (LDH) assay.