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. 2019 Jul 18;11(7):655. doi: 10.3390/v11070655

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of extracellular HBV DNA of sgtF1b and sgtF4 variants. HuH-7 cells were transfected with linear full-length HBV genomes of F1b wt, sgtF1b X(-), F1b X(-)+pF1bX, F1b/XF4, F1b/X(-)F4, F4 wt, F4 X(-), F4 X(-)+pF4X, F4/XF1b and F4/X(-)F1b variants. Ninety-six hours post-transfection, cell supernatants were harvest and HBV DNA was extracted and quantified by real-time PCR. As a control, the remaining input DNA was also quantified (0.70 ± 0.03 copies/mL), and this value was subtracted from those obtained for the viral variants. Results were expressed in relation to luciferase relative light units (RLU). Shown values represent the mean ± standard deviation of three independent experiments. *** p < 0.0001.