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. 2019 Jul 10;11(7):401. doi: 10.3390/toxins11070401

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evolutionary divergence tree of actinoporins from the genus Actinia. One cluster groups FraE, FraC, tenebrosin C (Ten-C), equinatoxin II (Eqt-II), and equinatoxin IV (Eqt-IV) and a second cluster groups FraB and equinatoxin V (Eqt-V). The length of the branches (number of substitutions per site expressed as parts per unit) determines the evolutionary distance from the common ancestor until speciation or until present. Confidence levels to assess the degree of relatedness of the proteins in a clade (bootstrap values) are shown next to the branches. The tree was built with MEGA X [34].