Table 1.
Physicochemical properties of formulations prepared with different ratios of low-molecular-weight hyaluronic acid/polyarginine (LMWHA/PArg) and evaluated in Milli-Q water (mean ± SD, n = 3).
| Mass Ratio LMWHA/PArg |
Charge Ratio [LMWHA]/[PArg] |
Size (nm) |
Polydispersity Index | Zeta Potential (mV) |
|---|---|---|---|---|
| 2.0/2.4 | 0.4 | 126 ± 29 | 0.3–0.4 | 21.6 ± 5 |
| 4.0/2.4 | 0.8 | 128 ± 2 | 0.1–0.2 | 24.35 ± 3 |
| 4.7/2.4 | 0.9 | 138 ± 23 | 0.1–0.2 | 23.3 ± 3 |
| 5.4/2.4 | 1.1 | Precipitation | --- | --- |
| 6.0/2.4 | 1.2 | 141 ± 10 | 0.1–0.2 | −17.9 ± 3 |
| 8.0/2.4 | 1.6 | 145 ± 9 | 0.2–0.3 | −33.1 ± 3 |
| 10/2.4 | 2.0 | 149 ± 32 | 0.2–0.3 | −35.0 ± 4 |
| 12/2.4 | 2.4 | 146 ± 18 | 0.2–0.3 | −36.2 ± 3 |
| 14/2.4 | 2.8 | 186 ± 71 | 0.1–0.2 | −39.7 ± 0.4 |
| 20/2.4 | 4.0 | 166 ± 13 | 0.1–0.2 | −42.6 ± 0.1 |