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Typical mass chromatograms of the liquid chromatography with tandem mass spectrometry (LC-MS/MS) obtained under multiple reaction monitoring (MRM) mode (m/z 320 > 162). MRM patterns of extracts from the flatworm Planocera multitentaculata egg plates spawned in the wild (A), extracts from the flatworm egg plates spawned in the aquarium (B), extracts from the wild adult flatworms (C), and 50 ng/mL tetrodotoxin (TTX) standard (D).
