Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 27;10(8):1692–1701. doi: 10.1111/1759-7714.13131

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

ZNF281 is a target of miR‐203. (a) Amount of circAGFG1 bound to SNRNP70 (positive control), Ago2 or IgG (negative control) detected by qPCR after RIP in A549 cells. (b) H1299 cells were transfected with biotinylated NC (Bio‐NC), biotinylated wild type miR‐203 (BiomiR‐203) or biotinylated mutant miR‐203 (Bio‐miR‐203‐mut), and biotin‐based miRNA pull‐down assays were performed 48 h after transfection. CircAGFG1 levels were analysed by qPCR. (c) Alignment of miR‐203 with the potential binding site of wild type ZNF281 and ZNF281 mutated at the potential binding site. (d) and (e) Luciferase reporter activity was measured in A549 and H1299 cells cotransfected with pPG‐miR‐203 (or empty vector as a control) and the luciferase empty vector (pmiR‐GLo) or with the vector containing wild type ZNF281 (pmiR‐GLo‐ZNF281‐wt) or mutant transcripts (pmiR‐GLo‐ZNF281‐mut). The relative ratio of firefly luciferase activity to Renilla luciferase activity is presented. All data are presented as mean ± s.d., and all experiments were performed at least three times. pPG‐miR‐NC, pPG‐miR‐203. *P < 0.05, ** < 0.01, *** < 0.001; n.s: not statistically significant.

Inline graphic — ZNF281 is a target of miR‐203. (a) Amount of circAGFG1 bound to SNRNP70 (positive control), Ago2 or IgG (negative control) detected by qPCR after RIP in A549 cells. (b) H1299 cells were transfected with biotinylated NC (Bio‐NC), biotinylated wild type miR‐203 (BiomiR‐203) or biotinylated mutant miR‐203 (Bio‐miR‐203‐mut), and biotin‐based miRNA pull‐down assays were performed 48 h after transfection. CircAGFG1 levels were analysed by qPCR. (c) Alignment of miR‐203 with the potential binding site of wild type ZNF281 and ZNF281 mutated at the potential binding site. (d) and (e) Luciferase reporter activity was measured in A549 and H1299 cells cotransfected with pPG‐miR‐203 (or empty vector as a control) and the luciferase empty vector (pmiR‐GLo) or with the vector containing wild type ZNF281 (pmiR‐GLo‐ZNF281‐wt) or mutant transcripts (pmiR‐GLo‐ZNF281‐mut). The relative ratio of firefly luciferase activity to Renilla luciferase activity is presented. All data are presented as mean ± s.d., and all experiments were performed at least three times. pPG‐miR‐NC, pPG‐miR‐203. *P < 0.05, ** < 0.01, *** < 0.001; n.s: not statistically significant.