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. 2019 Jun 27;10(8):1692–1701. doi: 10.1111/1759-7714.13131

Figure 5.

Figure 5

Silencing of ZNF281 inhibits A549 and H1299 cell proliferation and invasion. (a) Relative mRNA expression of ZNF281 was detected by qRT‐PCR in A549 and H1299 cells transfected with si‐NC or si‐ZNF281. (b) The protein expression of ZNF281, E‐cadherin, Vimentin and GAPDH was detected by Western blotting in A549 and H1299 cells transfected with si‐NC or si‐circAGFG1. (c) Transwell invasion assays in A549 and H1299 cell lines transfected with si‐NC or si‐ZNF281 were used to determine migration of A549 and H1299 cell lines. (d) Quantitative analysis of transwell invasion assays. (e) The colonizing ability of A549 and H1299 cells transfected with si‐NC or si‐ZNF281 was determined by using colony formation assays. All data are presented as mean ± s.d., and all experiments were performed at least three times. *P < 0.05, ** < 0.01, *** < 0.001; n.s: not statistically significant.