Skip to main content
. 2018 Jun 13;159(8):3090–3104. doi: 10.1210/en.2018-00146

Figure 2.

Figure 2.

mRNA levels of typical genes affected by seizures and HIF-1α staining in C57Bl/6J mice following 3 hours of SE. In all cases, SE was induced by pilocarpine administration, as in Fig. 1A. Bdnf mRNA levels in (A) hippocampus, (B) prefrontal cortex, and (C) amygdala measured by RT-qPCR and using Cyclo A as internal control (n = 9). Ngf mRNA levels in (D) hippocampus, (E) prefrontal cortex, and (F) amygdala measured by RT-qPCR and using Cyclo A as internal control (n = 9). Egr1 mRNA levels in (G) hippocampus, (H) prefrontal cortex, and (I) amygdala measured by RT-qPCR and using Cyclo A as internal control (n = 9). Ntf3 mRNA levels in (J) hippocampus, (K) prefrontal cortex, and (L) amygdala measured by RT-qPCR and using Cyclo A as internal control (n = 9). Values represent the mean ± SEM. (M–R) Low-power magnification images showing immunohistochemical analysis of HIF-1α in hippocampus (n = 4); (N, Q) ×100 and (O, R) ×200 magnifications of the CA1 field. (S) HIF-1α signal expressed per area in hippocampal CA1 field; values are mean ± SEM.