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Staining of A431 cells with MRIF with a protein-blocking step before incubation. A431 cell line xenograft frozen sections were blocked with 4% skimmed milk in (A) PBS or with (B) 10 ng/µl or (C) 60 ng/µl anti-EGFR neutralizing antibody overnight before MRIF staining. Scale bar = 100 μm. Abbreviations: PBS, phosphate buffered saline; EGFR, epidermal growth factor receptor; MRIF, magnetically promoted rapid immunofluorescence.
