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Immunohistochemistry in rat tibiae. (A–L) Collagen type I stains the bone matrix. (M–X) Osterix stains nuclei of osteoblasts. Black arrowheads indicate osterix-positive osteoblasts. (a–l) von Willebrand is a marker for vascular endothelial cells. Scale bars = 50 μm. Abbreviation: EDTA, ethylenediaminetetraacetic acid; HCl, hydrochloric acid.
