Table 1.

Monitoring Frequency for the Different Decalcification Conditions for Mouse and Rat Specimens.

Decalcification Solution/Temperature	Mouse				Rat
	10% EDTA, pH 7.4	10% Formic Acid	5% HCl	5% Nitric Acid	10% EDTA, pH 7.4	10% Formic Acid	5% HCl	5% Nitric Acid
4C	24 hr	4 hr	4 hr	2 hr	1 week	12 hr	4 hr	1 hr
25C	24 hr	4 hr	4 hr	2 hr	1 week	12 hr	4 hr	1 hr
37C	12 hr	2 hr	2 hr	1 hr	24 hr	6 hr	3 hr	30 min

Abbreviation: EDTA, ethylenediaminetetraacetic acid; HCl, hydrochloric acid.