. 2019 Jul 16;11(7):650. doi: 10.3390/v11070650

Table 1.

Primers and probes used for PCV screening and sequencing.

Primer Sets	Primer, Probe	Sequences	Accession Number	Position (nt–nt)	Length of the Amplicon (bp)	Reference
PCV3 screening	PCV3 Palinski_For	AGTGCTCCCCATTGAACG	KT869077	1427–1444	135	Palinski et al., 2016 [17]
	PCV3 Palinski_Rev	ACACAGCCGTTACTTCAC		1561–1544
	PCV3 Palinski_probe	[FAM]-ACCCCATGGCTCAACACATATGACC-[BHQ1]		1473–1449
Sequencing 2	PCV3 Palinski_For	AGTGCTCCCCATTGAACG		1427–1444	1007	Fux et al., 2018 [18]
Sequencing 2	PCV3 Pal Seq2_Rev	CGACCAAATCCGGGTAAGC		433–415	1007
Sequencing 5	PCV3 Pal Seq1_For	CACCGTGTGAGTGGATATAC		74–93	1072
Sequencing 5	PCV3 Fux 1144_Rev	CACCCCAACGCAATAATTGTA		1144–1124	1072
Sequencing 3	PCV3 Fux 1137_For	TTGGGGTGGGGGTATTTATT		1137–1156	425
Sequencing 3	PCV3 Palinski_Rev	ACACAGCCGTTACTTCAC		1561–1544	425
pGAPDH Set	pGAPDH_For	ACATGGCCTCCAAGGAGTAAGA	n/s	n/s	106	Duvigneau et al., 2005 [19]
	pGAPDH_Rev	GATCGAGTTGGGGCTGTGACT
	pGAPDH_probe	[HEX]CCACCAACCCCAGCAAGAGCACGC[BHQ1]
huGAPDH Set	huGAPDH_For	GGCGATGCTGGCGCTGAGTAC	AF261085	365–385	148	Behrendt et al., 2009 [20]
	huGAPDH_Rev	TGGTCCACACCCATGACGA		513–495
	huGAPDH_probe	[HEX]TTCACCACCATGGAGAAGGCTGGG[BHQ1]		407–430

PCV, Porcine circoviruses.