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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Anal Chem. 2019 Jun 12;91(13):8162–8169. doi: 10.1021/acs.analchem.9b00477

Figure 1.

Figure 1.

Affinity MS screen of 480-mix cocktails for ligand identification toward A2AR. (a) Experimental workflow. High- and low-affinity hits are distinguished by their MS intensity in the target vs control. (b) Affinity MS analysis of known A2AR ligands (red) and unrelated compouds (gray) in a simple mixture. Ligands with BI > 2 (n = 4) were identified. (c) Affinity MS screen of a 480-mix cocktail. Hits (mean BI > 2, P < 0.01, and n = 4) are indicated by red dots while gray dots represent negatives. (d) Hit identification from screening each 480-mix cocktail (M1−M10). (e) Total numbers of known and unreported ligands for A2AR identified from all 480-mix screens. Cpd, compound.