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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Anal Chem. 2019 Jun 12;91(13):8162–8169. doi: 10.1021/acs.analchem.9b00477

Figure 3.

Figure 3.

Affinity MS screen of 2400-mix and 4800-mix compound pools. (a) Modified workflow integrating iterative incubation of eluted compounds with the target. (b) Screening two 2400-mix pools and (c) one 4800-mix pool yielded the same set of initial hits. Initial hits and negatives are indicated by red and gray dots, respectively. (d) Overlap of hits from the 2400-mix and 4800-mix screens and comparison with the benchmark ligand set from the regular 480-mix screen. Isomeric compounds that cannot be distinguished by LC-MS/MS analysis are separately shown in panels b and c, yet they are de-duplicated to be a single hit in panel d. Cpd, compound.