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Affinity MS screens of a 20K compound pool. Hit identification from screening the 20K library with purified A2AR protein (a) or A2AR-expressing cell membranes (b). Descriptions for panels a and b are the same as those for Figure 3b,c. (c) Extracted LCMS traces of compound 3404 from the protein-based screen (left) or the membrane-based screen (right). (d) Overlap of hits from the two 20K-mix screens and comparison with the benchmark ligand set. Pro, protein; mem, membrane.
