Table 1-.
Sequence and reference information for qPCR analysis, with target genes, primer sequences, amplicon sizes and annealing temperatures
| Target Gene | Primer Sequence (5’−3’) | Amplicon size (bp) | Annealing Temperature | References | Control Strains |
|---|---|---|---|---|---|
| blaTEM | TEM_F: AAGATGCTGAAGATCA TEM_R: TTGGTATGGCTTCATTC |
425 | 44 °C | Speldooren et al., 1998 | E. coli T5 |
| blaSHV | SHV_F: GCGAAAGCCAGCTGTCGGGC SHV_R: GATTGGCGGCGCTGTTATCGC |
304 | 62 °C | Henriques et al., 2006a | E. coli 52 |
| blaCTX-M | CTX_F:58 SCVATGTGCAGYACCAGTAA CTX_R: G55CTGCCGGTYTTATCVCC |
652 | 55 °C | Lu et al., 2010 | E. coli 85.01 |
| blaIMP | IMP_F: GAATAGAGTGGCTTAATTGTC IMP_R: GGTTTAAYAAAACAACCACC |
232 | 55 °C | Henriques et al., 2006b | NTU 92/99 (IMP-1) and P. aeruginosa |
| blaVIM | VIM_F: GATGGTGTTTGGTCGCATATCG VIM_R: GCCACGTTCCCCGCAGACG |
475 | 58 °C | Henriques et al., 2006a | P. putida |
| blaKPC | KPC_F: CATTCAAGGGCTTTCTTGCTGC KPC_R: ACGACGGCATAGTCATTT |
538 | 55 °C | Dallenne et al, 2010 | E. coli USVAST-0600 |
| blaGES | GES_F: AGTCGGCTAGACCGGAAAG GES_R: TTTGTCCGTGCTCAGGAT |
399 | 57 °C | Dallenne et al., 2010 | E. coli GES |
| blaNDM |