KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Flag-M12 antibody | Sigma-Aldrich | Cat#. F1804 |
| Flag-M2 agarose | Sigma-Aldrich | Cat#. A2220 |
| Rabbit polyclonal anti-Tiam1 C16 | Santa-cruz biotechnology | Cat#. Sc-872 |
| Rabbit polyclonal anti-Tiam1 N15 | Santa-cruz biotechnology | Cat#. Sc-871 |
| Sheep polyclonal anti-Tiam1 | R&D systems | Cat#. AF-5038 |
| Mouse monoclonal anti-CaMKII (G-1) | Santa-cruz biotechnology | Cat#. Sc-5306 |
| Rabbit anti-phosphor-CaMKII T286 | Santa-cruz biotechnology | Cat#. Sc-12886 |
| Mouse monoclonal anti-Myc-9E10 antibody | Santa-cruz biotechnology | Cat#. Sc-40 |
| Mouse monoclonal anti-GST antibody (GS019) | Nacalai tesque | Cat#. 04435–26 |
| Mouse monoclonal anti-Rac1 antibody (clone 102) | BD biosciences | Cat#. 610651 |
| Mouse monoclonal anti-MAP2 antibody (HM2) | Novus Biologicals | Cat#. #NB120–11267 |
| Goat polyclonal anti-Kalirin-7 antibody | Novus Biologicals | Cat#. #NB100–41371 |
| Rabbit polyclonal anti-βPIX antibody | Cell Signaling technologies | Cat#. #4515S |
| Rabbit polyclonal anti-Phosphorylate Homer3 S120 | Mizutani et al. 2008 | N/A |
| Rabbit polyclonal anti- Phospho-(Ser/Thr) Akt Substrate Antibody | Cell Signaling technologies | Cat#. #9611S |
| Alexa647-goat anti-mouse IgG | Invitrogen | Cat#. A21236 |
| Alexa594-goat anti-rabbit IgG | Invitrogen | Cat#. A11037 |
| Alexa546-donkey anti-goat IgG | Invitrogen | Cat#. A11056 |
| Anti-mouse IgG HRP-linked whole Ab Sheep | GE healthcare | Cat#. NA931–1ML |
| Anti-rabbit IgG HRP-linked whole Ab donkey | GE healthcare | Cat#. NA934–1ML |
| Anti-sheep IgG HRP-linked | Invitrogen | Cat#. A16041 |
| Bacterial and Virus Strains | ||
| Lenti-FUGHW-shTiam1-Tiam1-Flag WT | This paper | N/A |
| Lenti-FUGHW-shTiam1-Tiam1-Flag AAA | This paper | N/A |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tetrodotoxin | Latoxan laboratory | Cat#. L8503 |
| Picrotoxin | Nacalai tesque | Cat#. 28004–71 |
| MNI-caged glutamate | Tocris | Cat#. 1490 |
| KN-93 | Tocris | Cat#. 1278 |
| EHT1864 | Tocris | Cat#. 3872 |
| AP-5 | Tocris | Cat#. 0106 |
| Phostag acrylamide | FUJIFILM Wako Pure Chemical Corporation | Cat#. AAL-107 |
| PEI max | Polyscience, Inc | Cat#. 24765–1 |
| Tiam1 CBD: RTLDSHASRMTQLKKQAALSG |
This paper | N/A |
| Tiam1 CBD scrambled: HLRMRLGSAKSQSTKLATADQ |
This paper | N/A |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Human: HEK293T cell | ATCC | CRL3216 |
| Experimental Models: Organisms/Strains | ||
| Rat; SD | SLC | |
| Oligonucleotides | ||
| Recombinant DNA | ||
| pmGFP-Rac1 | Hedrick et al., 2016 | N/A |
| pmCherry-PBD-mCherry | Hedrick et al., 2016 | N/A |
| pSuper-shTiam1 | Tolias et al., 2005 | N/A |
| pSuper-shKalirin-7 | Ma et al., 2008 | N/A |
| pmU6pro-sh-betaPIX | Saneyoshi et al., 2008 | N/A |
| pCAGGS-mGFP | This paper | N/A |
| pCAGGS-Tiam1-Flag | This paper | N/A |
| pCAGGS-Kalirin-7-Flag | This paper | Addgene plasmid#25454 |
| pCAGGS-betaPIX-Flag | Saneyoshi et al., 2008 | N/A |
| pCAGGS-Myc-CaMKI-alpha | Saneyoshi et al., 2008 | N/A |
| pCAGGS-Myc-CaMKII-alpha | Saneyoshi et al., 2008 | N/A |
| pCAGGS-Myc-CaMKIV | Saneyoshi et al., 2008 | N/A |
| pCAGGS-Myc-CaMKK-alpha | Saneyoshi et al., 2008 | N/A |
| pCAGGS-mCherry-CaMKII-alpha-mCherry | This paper | N/A |
| pCAGGS-Tiam1-EGFP | This paper | N/A |
| pCS2-Flag-Tiam1 1540–1591 | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc wild-type | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc F98K | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc E139R | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc I205K | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc W237K | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc K42R | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc T286A | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc T286D | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc K42R,T286D | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc T305DT306D | This paper | N/A |
| pCAGGS-CaMKII-alpha-myc 1–290 | This paper | N/A |
| pFHUGW-shLuc-LacZ-Flag | This paper | N/A |
| pFHUGW-shTiam1-LacZ-Flag | This paper | N/A |
| pFHUGW-shTiam1-Tiam1-Myc WT | This paper | N/A |
| pFHUGW-shTiam1-Tiam1-Myc AAA | This paper | N/A |
| p-mRFP1CaMKII-alpha-mGFP (camui) | Kwok et al., 2008 | N/A |
| pJPA-CaMKIIN | Chang et al., 1998 | N/A |
| pmCherry-PA-AIP2 | Murakoshi et al., 2016 | N/A |
| GST | pGEX4T1, GE healthcare | Cat#. 28954549 |
| GST-Tiam1 421–1591 | This paper | N/A |
| GST-Tiam1 421–852 | This paper | N/A |
| GST-Tiam1 840–1025 | This paper | N/A |
| GST-Tiam1 1025–1591 | This paper | N/A |
| GST-Tiam1 1540–1591 | This paper | N/A |
| Flag peptide | Sigma-Aldrich | Cat#. F3290 |
| Syntide2 | Calbiochem | Cat#. 05–23-4910 |
| AC3 | Anaspec Inc. | Cat#. 64927 |
| AIP | Anaspec Inc. | Cat#. 64926 |
| CaMKIINtide | Calbiochem | Cat#. 208920 |
| CBD | This paper | N/A |
| CBD scrambled | This paper | N/A |
| GST-Pak1RBD | Saneyoshi et al. 2008 | N/A |
| GST-Rac1 G15A | This paper | N/A |
| Lambda protein phosphatese | NEB | P0753S |
| GST-AC2 | This paper | N/A |
| GST-Homer3 | Mizutani et al. 2008 | N/A |
| Software and Algorithms | ||
| Igor Pro | Bosch et al., 2014 | https://www.wavemetrics.com/ |
| Statcel2 | OMS publishing Inc. | |
| KaleidaGraph | This paper | http://www.synergy.com/wordpress_650164087/kaleidagraph/ |
| ImageJ | Bosch et al., 2014 | https://imagej.nih.gov/nih-image/ |
| Other | ||