Table 2.
List of most informative proteins using hierarchical clustering in the cytoplasmic (cyt), secreted (sn) and nuclear (nuc) fraction upregulated in the resistant cells
| Fraction | Multiparameter in fractions/in all pellets | P‐value | log2FC | acc‐Nr | Protein name | Gene ID | Role in cancer and resistance |
|---|---|---|---|---|---|---|---|
| cyt | +/+ | 0.00095376 | −4.41 | P00352 | Retinal dehydrogenase 1 | ALDH1A1 | Stemness, disulfiram |
| +/− | 0.00014171 | −5.79 | P32119 | Peroxiredoxin‐2 | PRDX2 | Metastasis in melanoma | |
| +/+ | 8.77E‐05 | −6.19 | Q6NZI2 | Polymerase I and transcript release factor | PTRF | Metastasis, drug resistance | |
| +/− | 0.00038204 | −4.32 | O00151 | PDZ and LIM domain protein 1 | PDLIM1 | Metastasis breast cancer | |
| +/− | 6.06E‐07 | −5.94 | Q16270 | Insulin‐like growth factor‐binding protein 7 | IGFBP7 | EMT | |
| +/+ | 7.29E‐07 | −5.27 | P55809 | Succinyl‐CoA:3‐ketoacid‐coenzyme A transferase 1, mitochondrial | OXCT1 | Prostate cancer progression | |
| −/+ | 0.0121147 | −4.54 | Q9BZQ8 | Protein Niban | FAM129A | Survival mechanisms | |
| −/+ | 0.0189463 | −4.80 | P35237 | Serpin B6 | SERPINB6 | x | |
| −/+ | 0.0456127 | −4.48 | P47895 | Aldehyde dehydrogenase family 1 member A3 | ALDH1A3 | chemoresistance | |
| −/nd | 0.106551 | −4.41 | Q92771;Q96FC9;A8MPP1 | Putative ATP‐dependent RNA helicase DDX12 | DDX12P;DDX11;DDX11L8 | x | |
| −/+ | 0.00951598 | −4.29 | Q16719 | Kynureninase | KYNU | Active in cancer, Tryptophan | |
| −/+ | 0.0419105 | −5.76 | P09936 | Ubiquitin carboxyl‐terminal hydrolase isozyme L1 | UCHL1 | Associated with melanoma cell proliferation, MAPK | |
| 0.126862 | −6.03 | Q5XG92 | Carboxylesterase 4A | CES4A | Drug metabolism | ||
| sn | +/− | 3.02E‐10 | −7.70 | Q16270 | Insulin‐like growth factor‐binding protein 7 | IGFBP7 | |
| +/− | 0.00020224 | −6.55 | Q15113 | Procollagen C‐endopeptidase enhancer 1 | PCOLCE | Interaction with ECM | |
| +/− | 0.00024558 | −5.14 | P43490 | Nicotinamide phosphoribosyltransferase | NAMPT | Higher expression in melanoma, Tryptophan | |
| +/− | 5.89E‐07 | −5.08 | P14543 | Nidogen‐1 | NID1 | Higher expression in cancer, serum marker in breast cancer | |
| +/+ | 0.0105375 | −4.99 | P35442n | Thrombospondin‐2 | THBS2 | Higher expressed in melanoma metastasis | |
| nuc | +/+ | 6.76E‐05 | −7.93 | Q6NZI2 | Polymerase I and transcript release factor | PTRF | |
| +/− | 7.03E‐08 | −7.81 | Q16270 | Insulin‐like growth factor‐binding protein 7 | IGFBP7 |