FIGURE 5.

The abundance of RHEB-mTOR complex (A), GATOR2-Sestrin2 complex (B), Folliculin (C), and LRS (D) in longissimus dorsi muscle of 6- and 26-d-old pigs after 2-h infusion of saline (control), amino acids, or insulin. The values were normalized by β-actin abundance. White lines between bands indicate where images from the same blots were spliced to adjust sample order on the membrane for presentation. Values are least square means ± SEM; n = 4–6. Means with uncommon letters are significantly different: a > b > c. P < 0.05 was considered significant. AA, amino acid group; AU, arbitrary unit; GATOR2, GAP activity toward Rags 2; INS, insulin group; LRS, leucyl-tRNA synthetase; mTOR, mechanistic target of rapamycin; RHEB, Ras homolog enriched in brain; Sestrin2, stress response protein 2; Trt, treatment.