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. 2019 Jun 17;38(15):e100990. doi: 10.15252/embj.2018100990

Figure 3. ER stress modulates ATF6α–PDI mixed‐disulfide complexes.

Figure 3

  • A–D
    HEK293T cells were co‐transfected with ATF6α S1P and PDI substrate‐trapping mutants and either left untreated (−) or treated (+) for 1 h with 5 μM TG (A), 5 μg/ml tunicamycin (B), or 20 μM MG132 (C) to induce ER stress. ATF6α was immunoisolated from cell lysates with mouse anti‐ATF6α and separated by SDS–PAGE under non‐reducing conditions and ATF6α detected by Western blotting using rabbit anti‐HA. Blots indicate that mixed‐disulfide complexes between ATF6α and PDI enzymes are regulated by ER stress. M, D, and O refer to ATF6α monomer, dimer, and oligomer, respectively. Samples from (A) were rerun and probed with anti‐sera raised against respective PDI enzymes as indicated (D). * indicates positions of the ATF6α–PDI enzyme mixed‐disulfide complexes. ** indicates hypoglycosylated ATF6α. Data shown are representative of three independent experiments.

Source data are available online for this figure.