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. 2019 Aug;61(2):198–208. doi: 10.1165/rcmb.2018-0346OC

Figure 1.

Figure 1.

ESAT-6 (early secreted antigenic target protein 6) exacerbates granulomas and fibrosis in mice instilled with MWCNT or MWCNT + ESAT-6 or PBS for 60 days (60-day mice). (A) Representative light micrographs illustrating that granuloma formation in MWCNT + ESAT-6–instilled mice after 60 days is increased compared with mice instilled with MWCNT alone. As indicated by trichrome staining, fibrosis is increased in the areas around granulomas as well as within granulomas. Scale bars: 20 μm. (B) Modified Ashcroft scoring of fibrosis. In peroxisome proliferator–activated receptor γ–knockout (PPARγ–KO) mice 60 days after instillation with MWCNT + ESAT-6, fibrosis is increased compared with mice instilled with MWCNT alone and also compared with C57BL/6 wild-type mice instilled with MWCNT + ESAT-6. Reported are the mean ± SEM values of two independent observers from six lungs for each group. ★P ≤ 0.05. MWCNT = multiwall carbon nanotube.