Table 1.
Population | Murine Markers | Human Markers | References |
---|---|---|---|
CD4+ T cell | Low SSC, CD3ε+ or TCRαβ or γδ+, CD4+ | — | |
CD8+ T cell | Low SSC, CD3ε+ or TCRαβ or γδ+, CD8+ | — | |
CD4+ Treg cell | CD4+ T cell expressing Foxp3-transgenic fluorochrome, CD25hi | CD4+ T cell, CD127lo, CD25hi; intra-nuclear staining for FOXP3 | 10, 53, 54 |
Effector T cell | CD4+ or CD8+ T cell, CD62LloCD44hi, CD69hi | See below | 55, 56 |
Naive T cell | CD4+ or CD8+ T cell, CD62LhiCD44lo, CD69lo | CD4+ or CD8+ T cell, CCR7hiCD45RAhi | 55–60 |
Effector-memory T cell | — | CCR7loCD45RAlo | |
Central-memory T cell | — | CCR7hiCD45RAlo | |
T helper subset | Generally defined by transcription factor and cytokine staining (see text for examples) | — | |
CD4+ tissue-resident memory T cells | CD4+ T cell, CD11a+CD69+ | — | 61–64 |
CD8+ tissue-resident memory T cells | CD8+ T cell, CD103+CD69+ | — |
Definition of abbreviations: SSC = side scatter; Treg = regulatory T.