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. 2019 May 28;180(4):2022–2033. doi: 10.1104/pp.19.00020

Figure 3.

Figure 3.

AtERO1 and AtERO2 are required for plant growth under reducing conditions. A, Growth of AtERO1/2 mutant plants on medium containing DTT. Col-0, ero1-2, ero1-3, ero2-1, and ero2-2 plants were grown on one-half-strength MS medium with or without 2 mm DTT. Bar = 1 cm. B, Quantification of the fresh weight of 7-d-old Col-0, ero1-2, ero1-3, ero2-1, and ero2-2 plants. Data are shown as means ± se (n = 30). Statistical significance compared with Col-0 plants was analyzed using one-way ANOVA: **, P < 0.01; ns, nonsignificant. C, AtERO1 can complement the ero1-3 mutant. Col-0, ero1-3, and ProERO1:ERO1-HA/ero1-3 plants were grown on medium with or without 2 mm DTT for 7 d. Bar = 1 cm. D, Expression of AtERO1 proteins in ProERO1:ERO1-HA/ero1-3 transgenic plants. AtERO1-HA was detected by immunoblotting with an anti-HA antibody. Rubisco (RBC) stained with Coomassie Blue was used as a loading control.